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If your 2016 Honda Odyssey's USB port is malfunctioning, the first step is to restart the head unit. Sometimes, when the head unit freezes, the connected accessories may stop working. After resetting the head unit, inspect the USB port itself to ensure it is clean and undamaged. Insert the USB cable correctly and check the port's pins for any issues. Additionally, examine the wire harness at the back of the port to see if it's intact and secure. If the port still doesn't function properly after these steps, it may be necessary to replace it as it could be defective.
If hardware updates need to be reset, it could lead to the USB port not functioning in a 2016 Honda Odyssey.