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In the 2001 Ford F-350, the fuel pump relay is located in the fuse box, found by looking left of the steering wheel for a seam or crack along the dash. To access the fuse box, use a screwdriver or prybar to pop it open. Inside, there are numerous fuses. The back of the panel you opened will have a diagram showing the function of each fuse, or you can find this information in the owner's manual. Once you identify the needed fuse, simply pull it out. A good fuse will have a thin strip of metal connecting the two raised sections, while a bad fuse will have a broken strip. If the fuse is good, reinsert it; if not, replace it with a new one, which costs around $10.
The fuel pump relay for a 2001 Ford F-350 can be found at the center of the relay panel, positioned to the right of the trailer tow relay.